Modulation in vitro of H-ras oncogene expression by trans-splicing

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Antipain-induced suppression of oncogene expression in H-ras-transformed NIH3T3 cells.

Antipain (AP; 50 micrograms/ml) inhibits transformation of NIH3T3 cells after transfection with an activated H-ras oncogene. To determine whether AP effects on transformation are associated with alterations in oncogene expression, NIH3T3 cells were cotransfected with an activated H-ras oncogene and the selectable marker gene aph, and gene expression was quantified. Fifty percent of geneticin-re...

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Antipain-induced Suppression of Oncogene Expression in H-ras-transformed NIH3T3 Cells1

Antipain (AP; 50 Mg/ml) inhibits transformation of NIH3T3 cells after transfection with an activated H-ras oncogene. To determine whether AP effects on transformation are associated with alterations in oncogene expression, NIH3T3 cells were cotransfected with an activated H-ras oncogene and the selectable marker gene uph. and gene expression was quantified. Fifty percent of geneticin-resistant ...

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ژورنال

عنوان ژورنال: Oncogene

سال: 2001

ISSN: 0950-9232,1476-5594

DOI: 10.1038/sj.onc.1204473